recombinant histidine  (Sino Biological)

Journal: NPJ Science of Food

Article Title: The ENaC taste receptor’s perceived mechanism of mushroom salty peptides revealed by molecular interaction analysis

doi: 10.1038/s41538-025-00380-4

Figure Lengend Snippet: A – D thermodynamic interactions between peptides and the SCNN1α receptor; E – H thermodynamic interactions between peptides and the SCNN1β receptor; I – L thermodynamic interactions between peptides and the SCNN1γ receptor.

Article Snippet: Amiloride-sensitive sodium channel protein 1α recombinant protein (SCNN1α, sequence Tyr112 - Thr543, with N-terminal histidine tag), amiloride-sensitive sodium channel protein 1β recombinant protein (SCNN1β, sequence Tyr504 - Ile640, with N-terminal histidine and glutathione S-transferase tag), and amiloride-sensitive sodium channel protein 1γ recombinant protein (SCNN1γ, sequence Thr80 - Thr401, with N-terminal histidine tag) were expressed by prokaryotic expression (Host: E.coli) by Cloud-Clone.

Techniques:

Journal: NPJ Science of Food

Article Title: The ENaC taste receptor’s perceived mechanism of mushroom salty peptides revealed by molecular interaction analysis

doi: 10.1038/s41538-025-00380-4

Figure Lengend Snippet: Thermodynamic interaction results of salty peptide with ENaC taste receptor

Article Snippet: Amiloride-sensitive sodium channel protein 1α recombinant protein (SCNN1α, sequence Tyr112 - Thr543, with N-terminal histidine tag), amiloride-sensitive sodium channel protein 1β recombinant protein (SCNN1β, sequence Tyr504 - Ile640, with N-terminal histidine and glutathione S-transferase tag), and amiloride-sensitive sodium channel protein 1γ recombinant protein (SCNN1γ, sequence Thr80 - Thr401, with N-terminal histidine tag) were expressed by prokaryotic expression (Host: E.coli) by Cloud-Clone.

Techniques:

Journal: NPJ Science of Food

Article Title: The ENaC taste receptor’s perceived mechanism of mushroom salty peptides revealed by molecular interaction analysis

doi: 10.1038/s41538-025-00380-4

Figure Lengend Snippet: A Dynamic interactions between peptides and the SCNN1α receptor; B dynamic interactions between peptides and the SCNN1β receptor; C dynamic interactions between peptides and the SCNN1γ receptor.

Article Snippet: Amiloride-sensitive sodium channel protein 1α recombinant protein (SCNN1α, sequence Tyr112 - Thr543, with N-terminal histidine tag), amiloride-sensitive sodium channel protein 1β recombinant protein (SCNN1β, sequence Tyr504 - Ile640, with N-terminal histidine and glutathione S-transferase tag), and amiloride-sensitive sodium channel protein 1γ recombinant protein (SCNN1γ, sequence Thr80 - Thr401, with N-terminal histidine tag) were expressed by prokaryotic expression (Host: E.coli) by Cloud-Clone.

Techniques:

Journal: NPJ Science of Food

Article Title: The ENaC taste receptor’s perceived mechanism of mushroom salty peptides revealed by molecular interaction analysis

doi: 10.1038/s41538-025-00380-4

Figure Lengend Snippet: Dynamic interactions result of salty peptide with ENaC taste receptor

Article Snippet: Amiloride-sensitive sodium channel protein 1α recombinant protein (SCNN1α, sequence Tyr112 - Thr543, with N-terminal histidine tag), amiloride-sensitive sodium channel protein 1β recombinant protein (SCNN1β, sequence Tyr504 - Ile640, with N-terminal histidine and glutathione S-transferase tag), and amiloride-sensitive sodium channel protein 1γ recombinant protein (SCNN1γ, sequence Thr80 - Thr401, with N-terminal histidine tag) were expressed by prokaryotic expression (Host: E.coli) by Cloud-Clone.

Techniques:

Journal: NPJ Science of Food

Article Title: The ENaC taste receptor’s perceived mechanism of mushroom salty peptides revealed by molecular interaction analysis

doi: 10.1038/s41538-025-00380-4

Figure Lengend Snippet: Sequence information of ENaC taste receptor transmembrane structure

Article Snippet: Amiloride-sensitive sodium channel protein 1α recombinant protein (SCNN1α, sequence Tyr112 - Thr543, with N-terminal histidine tag), amiloride-sensitive sodium channel protein 1β recombinant protein (SCNN1β, sequence Tyr504 - Ile640, with N-terminal histidine and glutathione S-transferase tag), and amiloride-sensitive sodium channel protein 1γ recombinant protein (SCNN1γ, sequence Thr80 - Thr401, with N-terminal histidine tag) were expressed by prokaryotic expression (Host: E.coli) by Cloud-Clone.

Techniques: Sequencing

Journal: NPJ Science of Food

Article Title: The ENaC taste receptor’s perceived mechanism of mushroom salty peptides revealed by molecular interaction analysis

doi: 10.1038/s41538-025-00380-4

Figure Lengend Snippet: A – D thermodynamic interactions between peptides and the SCNN1α receptor; E – H thermodynamic interactions between peptides and the SCNN1β receptor; I – L thermodynamic interactions between peptides and the SCNN1γ receptor.

Article Snippet: Amiloride-sensitive sodium channel protein 1α recombinant protein (SCNN1α, sequence Tyr112 - Thr543, with N-terminal histidine tag), amiloride-sensitive sodium channel protein 1β recombinant protein (SCNN1β, sequence Tyr504 - Ile640, with N-terminal histidine and glutathione S-transferase tag), and amiloride-sensitive sodium channel protein 1γ recombinant protein (SCNN1γ, sequence Thr80 - Thr401, with N-terminal histidine tag) were expressed by prokaryotic expression (Host: E.coli) by Cloud-Clone.

Techniques:

Journal: NPJ Science of Food

Article Title: The ENaC taste receptor’s perceived mechanism of mushroom salty peptides revealed by molecular interaction analysis

doi: 10.1038/s41538-025-00380-4

Figure Lengend Snippet: Thermodynamic interaction results of salty peptide with ENaC taste receptor

Article Snippet: Amiloride-sensitive sodium channel protein 1α recombinant protein (SCNN1α, sequence Tyr112 - Thr543, with N-terminal histidine tag), amiloride-sensitive sodium channel protein 1β recombinant protein (SCNN1β, sequence Tyr504 - Ile640, with N-terminal histidine and glutathione S-transferase tag), and amiloride-sensitive sodium channel protein 1γ recombinant protein (SCNN1γ, sequence Thr80 - Thr401, with N-terminal histidine tag) were expressed by prokaryotic expression (Host: E.coli) by Cloud-Clone.

Techniques:

Journal: NPJ Science of Food

Article Title: The ENaC taste receptor’s perceived mechanism of mushroom salty peptides revealed by molecular interaction analysis

doi: 10.1038/s41538-025-00380-4

Figure Lengend Snippet: A Dynamic interactions between peptides and the SCNN1α receptor; B dynamic interactions between peptides and the SCNN1β receptor; C dynamic interactions between peptides and the SCNN1γ receptor.

Article Snippet: Amiloride-sensitive sodium channel protein 1α recombinant protein (SCNN1α, sequence Tyr112 - Thr543, with N-terminal histidine tag), amiloride-sensitive sodium channel protein 1β recombinant protein (SCNN1β, sequence Tyr504 - Ile640, with N-terminal histidine and glutathione S-transferase tag), and amiloride-sensitive sodium channel protein 1γ recombinant protein (SCNN1γ, sequence Thr80 - Thr401, with N-terminal histidine tag) were expressed by prokaryotic expression (Host: E.coli) by Cloud-Clone.

Techniques:

Journal: NPJ Science of Food

Article Title: The ENaC taste receptor’s perceived mechanism of mushroom salty peptides revealed by molecular interaction analysis

doi: 10.1038/s41538-025-00380-4

Figure Lengend Snippet: Dynamic interactions result of salty peptide with ENaC taste receptor

Article Snippet: Amiloride-sensitive sodium channel protein 1α recombinant protein (SCNN1α, sequence Tyr112 - Thr543, with N-terminal histidine tag), amiloride-sensitive sodium channel protein 1β recombinant protein (SCNN1β, sequence Tyr504 - Ile640, with N-terminal histidine and glutathione S-transferase tag), and amiloride-sensitive sodium channel protein 1γ recombinant protein (SCNN1γ, sequence Thr80 - Thr401, with N-terminal histidine tag) were expressed by prokaryotic expression (Host: E.coli) by Cloud-Clone.

Techniques:

Journal: NPJ Science of Food

Article Title: The ENaC taste receptor’s perceived mechanism of mushroom salty peptides revealed by molecular interaction analysis

doi: 10.1038/s41538-025-00380-4

Figure Lengend Snippet: Sequence information of ENaC taste receptor transmembrane structure

Article Snippet: Amiloride-sensitive sodium channel protein 1α recombinant protein (SCNN1α, sequence Tyr112 - Thr543, with N-terminal histidine tag), amiloride-sensitive sodium channel protein 1β recombinant protein (SCNN1β, sequence Tyr504 - Ile640, with N-terminal histidine and glutathione S-transferase tag), and amiloride-sensitive sodium channel protein 1γ recombinant protein (SCNN1γ, sequence Thr80 - Thr401, with N-terminal histidine tag) were expressed by prokaryotic expression (Host: E.coli) by Cloud-Clone.

Techniques: Sequencing

Journal: Antibodies

Article Title: Functional Activity of Cytokine-Induced Killer Cells Enhanced by CAR-CD19 Modification or by Soluble Bispecific Antibody Blinatumomab

doi: 10.3390/antib13030071

Figure Lengend Snippet: CAR structures. Schematic representation of the two anti-CD19 CAR structures used. Images created with Biorender.com .

Article Snippet: For in vitro functional assays, ten days after transfection the anti-CD19 CAR + cells were purified by labeling with the poly-histidine tagged recombinant human CD19 protein (His-rhCD19, Acro biosystems, Newark, DE, USA), followed by the anti-histidine FITC antibody and immunoselected through an anti-FITC magnetic beads separation column (Miltenyi Biotec, Bergisch, Gladbach, Germany).

Techniques:

Journal: Antibodies

Article Title: Functional Activity of Cytokine-Induced Killer Cells Enhanced by CAR-CD19 Modification or by Soluble Bispecific Antibody Blinatumomab

doi: 10.3390/antib13030071

Figure Lengend Snippet: Characterization of CARCIK-CD19 cells. ( A ) PBMCs were transfected with the CAR-MNZ and CAR-BG2 plasmids and expanded to CIK for 21 days. Total cell number at the end of the culture is shown, starting from the same number of cells in both cases (10 × 10 6 cells). ( B , C ) Percentage ( B ) and MFI ( C ) of CAR expression on CD3 + cells at the end of culture. ( D , E ) Immunophenotype was analyzed at the end of the cultures (day 21) by flow cytometry, including percentages of CD3 + , CD3 + CD4 + , CD3 + CD8 + , CD3 + CD56 + ( D ) and effector memory populations ( E ); ( F – H ) CAR + cells were purified at day 10–14 by immunoselection. Percentages of CAR + cells pre- and post-purification are shown. Representative flow cytometry histograms of CAR expression of non-purified and purified CARCIK-CD19 (respectively, CAR-MNZ 26.6%, 4588 MFI, and 97.1%, 4031 MFI; CAR-BG2 20.4%, 2641 MFI, and 96.8%, 3208 MFI). The results are the means and standard deviations of four to five experiments using different donors as starting material. (*: p < 0.05, ns: not significant).

Article Snippet: For in vitro functional assays, ten days after transfection the anti-CD19 CAR + cells were purified by labeling with the poly-histidine tagged recombinant human CD19 protein (His-rhCD19, Acro biosystems, Newark, DE, USA), followed by the anti-histidine FITC antibody and immunoselected through an anti-FITC magnetic beads separation column (Miltenyi Biotec, Bergisch, Gladbach, Germany).

Techniques: Transfection, Expressing, Flow Cytometry, Purification

Journal: Antibodies

Article Title: Functional Activity of Cytokine-Induced Killer Cells Enhanced by CAR-CD19 Modification or by Soluble Bispecific Antibody Blinatumomab

doi: 10.3390/antib13030071

Figure Lengend Snippet: In vivo activity. ( A ) Kaplan–Meier survival curves of NSG mice engrafted with Daudi cells, left untreated (DAUDI only), or treated with CIKs, alone or receiving blinatumomab for the first three weeks, CARCIK-MNZ and CARCIK-BG2. ( B ) Analysis of hCD45 + CD3 + cells/mL in the PB of animals treated with the different effectors. The data with DAUDI only condition (<63 hCD45 + CD19 + /mL) are not inserted in the graph for major clarity. ( C ) Analysis of hCD45 + CD19 + cells/mL in the PB of animals treated with the different effectors or left untreated. ( D ) Percentages of hCD45 + CD3 + cells in the BM, spleen, and kidney. ( E ) Percentages of hCD45 + CD19 + cells in the BM, spleen, and kidney. ( F ) Mice body weight after the treatment. (** p < 0.01, *** p < 0.001, ns: not significant).

Article Snippet: For in vitro functional assays, ten days after transfection the anti-CD19 CAR + cells were purified by labeling with the poly-histidine tagged recombinant human CD19 protein (His-rhCD19, Acro biosystems, Newark, DE, USA), followed by the anti-histidine FITC antibody and immunoselected through an anti-FITC magnetic beads separation column (Miltenyi Biotec, Bergisch, Gladbach, Germany).

Techniques: In Vivo, Activity Assay